Hialid 0.1/Hialid 0.3/Hialid Mini 0.3 Mechanism of Action

Pharmacotherapeutic group: Artificial tears and other indifferent formulations. ATC code: S01XA20.
Pharmacology: Pharmacodynamics: Mechanism of action: Sodium hyaluronate binds to fibronectin and accelerates the adhesion and migration of corneal epithelial cells. Sodium hyaluronate also has an excellent water-retentive property because each sodium hyaluronate molecule can retain many H₂O molecules.
Acceleration of corneal wound healing: When 0.1% - 0.5% ophthalmic solutions of sodium hyaluronate were instilled in rabbit wound models with corneal epithelium and corneal epithelial basement membrane detached, the wounded area decreased significantly compared to vehicle ophthalmic solution from 24 hours post-detachment and onwards.
Acceleration of corneal epithelial migration: Sodium hyaluronate significantly accelerated the migration of corneal epithelial layer compared to the control group (culture medium only) in isolated strips of cultured rabbit cornea.
Water retentive property: When 0.1% - 1.0% sodium hyaluronate solutions were dropped onto agar plugs, weight loss of agar plugs due to water evaporation was prevented in a concentration-dependently manner.
Pharmacokinetics: Serum concentration: Serum concentrations of hyaluronic acid were determined before topical application as well as on treatment day 3, day 9 (the last day of dosing), and day 10 in 6 healthy adult male volunteers. To the unilateral eye of the subjects, 0.1% (day 1) and 0.5% (day 2 - 9) ophthalmic solutions of sodium hyaluronate were instilled at a dose of one drop 5 times daily (day 1 - 2) and one drop 13 times daily (day 3 - 9). All serum concentrations determined before, during, and after treatment were less than the limit of quantitation (10 µg/mL).
Intraocular distribution: (rabbits): Following a single topical application of 50 µL of 0.1% ¹⁴C-sodium hyaluronate ophthalmic solution in rabbits with a normal cornea, radioactivity was only detected in the outer ocular area. Especially, the radioactivity in the bulbar conjunctiva was high and it was detectable till at least 8 hours after application. In contrast, the radioactivity in the cornea was low and it was only detectable for 0.5 hour after application.
Following topical application of 50 µL of 0.1% ¹⁴C-sodium hyaluronate ophthalmic solution in rabbits with corneal epithelium wound, approximately 407.5 ng eq. of HA/g of the concentration was quantitated in the cornea even at 1 hour after application, indicating better penetration of ¹⁴C-radioactivity into the wound cornea. Radioactivity was also detected in the aqueous humor.